yvqK

168

ATP:cob(I)alamin adenosyltransferase

locus: BSU_33150

Molecular weight: 21.34 kDa

pI: 5.21

Protein length: 193 aa

Gene length: 582 bp

function: unknown

product: ATP:cob(I)alamin adenosyltransferase

essential: no

ec: 2.5.1.17

synonyms: yvqK

List of homologs in different organisms, belongs to COG2096 (Galperin et al., 2021)

Gene

Coordinates: 3,400,537  3,401,118

The protein

Catalyzed reaction/ biological activity

• 2 ATP + 2 cob(II)yrinate a,c diamide + reduced [electron-transfer flavoprotein] --> 2 adenosylcob(III)yrinate a,c-diamide + 3 H⁺ + oxidized [electron-transfer flavoprotein] + 2 triphosphate (according to UniProt)
• 2 ATP + 2 cob(II)alamin + reduced [electron-transfer flavoprotein] --> 2 adenosylcob(III)alamin + 3 H⁺ + oxidized [electron-transfer flavoprotein] + 2 triphosphate (according to UniProt)

Protein family

• Cob(I)alamin adenosyltransferase family (single member, according to UniProt)

Structure

• [PDB|1RTY]
• [AF|O34899]

Expression and Regulation

Operons

• genes: [gene|C76265F521D9A153F28AC6BACD935CC20DEA5D9C|yvrC]-[gene|1DC4A3D7E93677B0EAD15F43C80E50FD98527EBC|yvrB]-[gene|128EBAE9C14C7BB75173DF912853A6FCE86ABCE4|yvrA]-[gene|468D9141EAC78556C9F6C564875F70EC43E60C1C|yvqK]

  regulation

  • expression is decreased in the presence of cobalamin (vitamin B12) ([wiki|B12 riboswitch]) [Pubmed|14704351]

  regulatory mechanism

  • B12 riboswitch: RNA switch, upon binding of B12 [Pubmed|14704351], in [regulon|other_regulator:B12 riboswitch|B12 riboswitch]

Biological materials

Mutant

• MGNA-B042 (yvqK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1041 NBRP B. subtilis, Japan]
• BKE33150 ([gene|468D9141EAC78556C9F6C564875F70EC43E60C1C|yvqK]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33150 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATACCCTGAATCTCCTTTA,  downstream forward: _UP4_TAAAAAGTGAAAAGGAAAGC
• BKK33150 ([gene|468D9141EAC78556C9F6C564875F70EC43E60C1C|yvqK]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33150 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATACCCTGAATCTCCTTTA,  downstream forward: _UP4_TAAAAAGTGAAAAGGAAAGC

References

• 17588214,14704351