yvqK

168

ATP:cob(I)alamin adenosyltransferase

Locus: BSU_33150

Molecular weight: 21.34 kDa

Isoelectric point: 5.21

Protein length: 193 aa

Gene length: 582 bp

Function: unknown

Product: ATP:cob(I)alamin adenosyltransferase

Essential: no

E.C.: 2.5.1.17

Synonyms: yvqK

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2096 (Galperin et al., 2021)

Gene

Coordinates: 3,400,537  3,401,118

The protein

Catalyzed reaction/ biological activity

• 2 ATP + 2 cob(II)yrinate a,c diamide + reduced [electron-transfer flavoprotein] --> 2 adenosylcob(III)yrinate a,c-diamide + 3 H⁺ + oxidized [electron-transfer flavoprotein] + 2 triphosphate (according to UniProt)
• 2 ATP + 2 cob(II)alamin + reduced [electron-transfer flavoprotein] --> 2 adenosylcob(III)alamin + 3 H⁺ + oxidized [electron-transfer flavoprotein] + 2 triphosphate (according to UniProt)

Protein family

• Cob(I)alamin adenosyltransferase family (single member, according to UniProt)

Structure

• 1RTY (PDB)
• O34899 (AlphaFold)

Expression and Regulation

Operons

• Genes: yvrC-yvrB-yvrA-yvqK

  Regulation

  • expression is decreased in the presence of cobalamin (vitamin B12) (B12 riboswitch) PubMed

  Regulatory mechanism

  • B12 riboswitch: RNA switch, upon binding of B12 PubMed, in B12 riboswitch

Biological materials

Mutant

• MGNA-B042 (yvqK::erm), available at the NBRP B. subtilis, Japan
• BKE33150 (yvqK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACCCTGAATCTCCTTTA,  downstream forward: _UP4_TAAAAAGTGAAAAGGAAAGC
• BKK33150 (yvqK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACCCTGAATCTCCTTTA,  downstream forward: _UP4_TAAAAAGTGAAAAGGAAAGC

References

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