liaS

168

two-component sensor kinase/phosphatase, response to bacitracin

Locus: BSU_33090

Molecular weight: 40.55 kDa

Isoelectric point: 7.33

Protein length: 360 aa

Gene length: 1083 bp

Function: control of LiaR activity in response to bacitracin

Product: two-component sensor kinase

Essential: no

Synonyms: liaS, yvqE

Genomic Context

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List of homologs in different organisms, belongs to COG4585 (Galperin et al., 2021)

Gene

Coordinates: 3,395,035  3,396,117

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of LiaR
• dephosphorylation of LiaR in the absence of the stress signal PubMed
• ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

Domains

• two transmembrane segments
• HAMP domain (aa 74-126) (according to UniProt)
• Histidine kinase domain (aa 153-346) (according to UniProt)

Structure

• 3GIG (PDB) (DesK, corresponds to the C-terminal domain, aa 149 ... 343, 25% identity) PubMed
• O32198 (AlphaFold)

Modification

• autophosphorylation on a His residue

Effectors of protein activity

• LiaS kinase activity is inhibited by LiaF, phosphatase acitivity is maintained by LiaF in the absence of the stress signal PubMed

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: liaG-liaF-liaS-liaR

  Description: PubMed

  Regulation

  • ''liaG'': constitutive

  Regulatory mechanism

  • LiaR: activation, PubMed, in liaR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: liaI-liaH-liaG-liaF-liaS-liaR

  Description: PubMed

  Regulation

  • ''liaG'': constitutive

Biological materials

Mutant

• MGNA-B036 (yvqE::erm), available at the NBRP B. subtilis, Japan
• BKE33090 (liaS::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GAGGCTGGCAAGCATTTTTT,  downstream forward: _UP4_CCGGAAGAAAAAGGAGAGAA
• BKK33090 (liaS::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GAGGCTGGCAAGCATTTTTT,  downstream forward: _UP4_CCGGAAGAAAAAGGAGAGAA

Labs working on this gene/protein

• John Helmann, Cornell University, USA Homepage

References

Reviews

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Original Publications

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