yirB

168

anti-adaptor protein, inhibits SpxH, responsible for stabilization of Spx in response to cell wall stress

Locus: BSU_33029

Molecular weight: 6.60 kDa

Isoelectric point: 9.931

Protein length: 54 aa

Gene length: 165 bp

Function: control of Spx proteolysis

Product: anti-adaptor protein

Essential: no

Synonyms: yirB, yuzO

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 3,387,781  3,387,945

The protein

Catalyzed reaction/ biological activity

• prevention of the SpxH-Spx interaction PubMed

Structure

• 5BRQ (PDB) (from B. licheniformis, 29% identity) PubMed
• O32302 (AlphaFold)

Expression and Regulation

Operons

• Genes: yirB

  Description: PubMed

  Regulation

  • induced by vancomycin (CssR-P) PubMed
  • induced by cell wall stress (CssR) PubMed

  Regulatory mechanism

  • YuxN: repression, PubMed, in yuxN regulon
  • CssR: activation, CssR-P acts as anti-repressor PubMed, in cssR regulon

Biological materials

Mutant

• BKE33029 (yirB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTAGATTCACCCTTTC,  downstream forward: _UP4_TAAAAAAATAGACTGCAAAA
• BKK33029 (yirB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTAGATTCACCCTTTC,  downstream forward: _UP4_TAAAAAAATAGACTGCAAAA

References

Reviews

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Research papers

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