yusF

168

unknown

Locus: BSU_32780

Molecular weight: 16.75 kDa

Isoelectric point: 6.6

Protein length: 146 aa

Gene length: 441 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yusF

Genomic Context

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List of homologs in different organisms, belongs to COG1658 (Galperin et al., 2021)

Gene

Coordinates: 3,365,391 → 3,365,831

The protein

Domains

• TOPRIM domain (aa 31-119) PubMed

Structure

• 2I5R (PDB) (small TOPRIM domain in complex with Mg2+, Geobacillus stearothermophilus) PubMed
• 2FCJ (PDB) (small TOPRIM domain, Geobacillus stearothermophilus) PubMed
• O32172 (AlphaFold)

Biological materials

Mutant

• MGNA-B591 (yusF::erm), available at the NBRP B. subtilis, Japan
• BKE32780 (ΔyusF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GGCAGTGAAATTCACCGCCA,  downstream forward: _UP4_GAAAGAAAGTCCCGAGGTGT
• BKK32780 (ΔyusF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GGCAGTGAAATTCACCGCCA,  downstream forward: _UP4_GAAAGAAAGTCCCGAGGTGT

References

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