metP

168

methionine ABC transporter, permease

Locus: BSU_32740

Molecular weight: 23.61 kDa

Isoelectric point: 6.62

Protein length: 222 aa

Gene length: 669 bp

Function: methionine uptake

Product: methionine ABC transporter, permease

Essential: no

Synonyms: metP, yusB

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2011 (Galperin et al., 2021)

Gene

Coordinates: 3,362,605 → 3,363,273

The protein

Catalyzed reaction/ biological activity

• uptake of methionine

Protein family

• Binding-protein-dependent transport system permease family (according to UniProt)
• CysTW subfamily (according to UniProt)

Domains

• ABC transmembrane type-1 domain (aa 18-212) (according to UniProt)

Structure

• 3DHW (PDB) (the MetN-MetP complex from E. coli, 44% identity) PubMed
• O32168 (AlphaFold)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: metN-metP-metQ

  Description: PubMed

  Regulation

  • activated during growth in the presence of branched chain amino acids (CodY) PubMed
  • the mRNA is processed between metP and metQ by RNase Y, this requires the RicA-RicF-RicT complex PubMed
  • the S-box RNA is degraded by RNase Y PubMed

  Regulatory mechanism

  • S-box: RNA switch, the S-box riboswitch binds S-adenosylmethionine resulting in termination, in S-box
  • CodY: activation, PubMed, in codY regulon

Biological materials

Mutant

• MGNA-A600 (yusB::erm), available at the NBRP B. subtilis, Japan
• 1A797 ( metP::cat), PubMed, available at BGSC
• BKE32740 (ΔmetP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTCAAACATTCGTGATCAC,  downstream forward: _UP4_GACAAACGATAAGGGAGGAT
• BKK32740 (ΔmetP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTCAAACATTCGTGATCAC,  downstream forward: _UP4_GACAAACGATAAGGGAGGAT

References

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