pucF

168

allantoate amidohydrolase

Locus: BSU_32530

Molecular weight: 45.36 kDa

Isoelectric point: 5.36

Protein length: 412 aa

Gene length: 1239 bp

Function: purine utilization

Product: allantoate amidohydrolase

Essential: no

Synonyms: pucF, yurH

Genomic Context

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Categories containing this gene/protein

Metabolism, Nucleotide metabolism, Utilization of nucleotides

List of homologs in different organisms, belongs to COG0624 (Galperin et al., 2021)

Gene

Coordinates: 3,342,433 → 3,343,671

The protein

Catalyzed reaction/ biological activity

allantoate + 2 H⁺ + H₂O --> (S)-2-ureidoglycine + CO₂ + NH₄⁺ (according to UniProt)

Protein family

peptidase M20 family (according to UniProt)

Structure

2IMO (PDB) (from Escherichia coli k12, 47% identity, 63% similarity) PubMed
O32149 (AlphaFold)

Expression and Regulation

Operons

Genes: pucF-pucG

Description: PubMed

Regulation

induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed

Expression

expression is heterogeneous PubMed

Regulatory mechanism

PucR: activation, PubMed, in pucR regulon

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

MGNA-A943 (yurH::erm), available at the NBRP B. subtilis, Japan
BKE32530 (ΔpucF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTACTCCCCCCTCTGGC,  downstream forward: _UP4_GCTTACTGATAAAGGAGGAA
BKK32530 (ΔpucF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTACTCCCCCCTCTGGC,  downstream forward: _UP4_GCTTACTGATAAAGGAGGAA

References

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