pucC

168

xanthine dehydrogenase

Locus: BSU_32490

Molecular weight: 29.97 kDa

Isoelectric point: 8.58

Protein length: 277 aa

Gene length: 834 bp

Function: purine utilization

Product: xanthine dehydrogenase

Essential: no

Synonyms: pucC, yurD

Genomic Context

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List of homologs in different organisms, belongs to COG1319 (Galperin et al., 2021)

Gene

Coordinates: 3,338,501 → 3,339,334

RNA

Catalyzed reaction/ biological activity

• H₂O + NAD⁺ + xanthine --> H⁺ + NADH + urate (according to UniProt)
• H₂O + hypoxanthine + NAD⁺ --> H⁺ + NADH + xanthine (according to UniProt)

The protein

Catalyzed reaction/ biological activity

• Xanthine   NAD   H₂O = urate   NADH (according to Swiss-Prot)

Domains

• FAD-binding PCMH-type domain (aa 7-176) (according to UniProt)

Cofactors

• FAD (according to UniProt)

Structure

• 1JRO (PDB) (from Rhodobacter capsulatus, 39% identity, 55% similarity) PubMed
• O32145 (AlphaFold)

Expression and Regulation

Operons

• Genes: pucA-pucB-pucC-pucD-pucE

  Description: PubMed

  Regulation

  • induced in the presence of purine nucleotides (effector: allantoin) (PucR) PubMed

  Regulatory mechanism

  • PucR: activation, PubMed, in pucR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A940 (yurD::erm), available at the NBRP B. subtilis, Japan
• BKE32490 (ΔpucC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CCTTGCTTTTGTCACTTGGC,  downstream forward: _UP4_CTGATGGCTGAGGGAGGGGA
• BKK32490 (ΔpucC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CCTTGCTTTTGTCACTTGGC,  downstream forward: _UP4_CTGATGGCTGAGGGAGGGGA

References

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