pucE

168

xanthine dehydrogenase

Locus: BSU_32470

Molecular weight: 18.69 kDa

Isoelectric point: 6.13

Protein length: 173 aa

Gene length: 522 bp

Function: purine utilization

Product: xanthine dehydrogenase

Essential: no

Synonyms: pucE, yurB

Genomic Context

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List of homologs in different organisms, belongs to COG2080 (Galperin et al., 2021)

Gene

Coordinates: 3,335,751  3,336,272

The protein

Catalyzed reaction/ biological activity

• H₂O + NAD⁺ + xanthine --> H⁺ + NADH + urate (according to UniProt)
• H₂O + hypoxanthine + NAD⁺ --> H⁺ + NADH + xanthine (according to UniProt)

Domains

• 2Fe-2S ferredoxin-type domain (aa 14-90) (according to UniProt)

Cofactors

• Fe-S cluster PubMed

Structure

• 1JRO (PDB) (from Rhodobacter capsulatus, 43% identity, 58% similarity) PubMed
• O32143 (AlphaFold)

Expression and Regulation

Operons

• Genes: pucA-pucB-pucC-pucD-pucE

  Description: PubMed

  Regulation

  • induced in the presence of purine nucleotides (effector: allantoin) (PucR) PubMed

  Regulatory mechanism

  • PucR: activation, PubMed, in pucR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A939 (yurB::erm), available at the NBRP B. subtilis, Japan
• BKE32470 (pucE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TACAGGAAATGGCCCGGCCT,  downstream forward: _UP4_TAAAAGCCGGACTTTTTTAG
• BKK32470 (pucE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TACAGGAAATGGCCCGGCCT,  downstream forward: _UP4_TAAAAGCCGGACTTTTTTAG

References

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