yumB

168

similar to type II NADH dehydrogenase (non proton-pumping)

Locus: BSU_32100

Molecular weight: 44.77 kDa

Isoelectric point: 6.62

Protein length: 406 aa

Gene length: 1221 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yumB

Genomic Context

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List of homologs in different organisms, belongs to COG1252 (Galperin et al., 2021)

Gene

Coordinates: 3,300,034  3,301,254

The protein

Protein family

• NADH dehydrogenase family (with YutJ and Ndh, according to UniProt)

Cofactors

• FAD (according to UniProt)

Structure

• 4NWZ (PDB) (from Caldalkalibacillus thermarum, 62% identity) PubMed
• O05267 (AlphaFold)

Paralogous protein(s)

• Ndh

Expression and Regulation

Operons

• Genes: yumB

  Description: PubMed

Biological materials

Mutant

• MGNA-B571 (yumB::erm), available at the NBRP B. subtilis, Japan
• BKE32100 (yumB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCACATCCACCTTCTCTT,  downstream forward: _UP4_TAAAAACCCGCGGAAGCGGG
• BKK32100 (yumB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCACATCCACCTTCTCTT,  downstream forward: _UP4_TAAAAACCCGCGGAAGCGGG

References

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