ybdZ

168

unknown

Locus: BSU_31959

Molecular weight: 7.90 kDa

Isoelectric point: 4.484

Protein length: 69 aa

Gene length: 210 bp

Function: unknown

Product: unknown

Essential: -

Synonyms: ybdZ

Genomic Context

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List of homologs in different organisms, belongs to COG3251 (Galperin et al., 2021)

Gene

Coordinates: 3,280,294 → 3,280,503

The protein

Structure

• 5U89 (PDB) (from Geobacillus sp., 66% identity) PubMed
• C0H3Q6 (AlphaFold)

Expression and Regulation

Operons

• Genes: dhbA-dhbC-dhbE-dhbB-dhbF-ybdZ

  Description: PubMed

  Regulation

  • induced by iron starvation (second wave to allow iron scavenging from the environment) (Fur) PubMed
  • expression is increased in the presence of pulchimerrin PubMed

  Expression

  • expression is heterogeneous PubMed

  Regulatory mechanism

  • Fur: repression, PubMed, in fur regulon
  • AbrB: repression, PubMed, in abrB regulon
  • Kre: repression, in kre regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigI: sigma factor, PubMed, in sigI regulon

  Additional information

  • the ''dhbA-dhbC-dhbE-dhbB-dhbF operon is strongly unregulated in a kre'' mutant PubMed

Biological materials

Mutant

• BKE31959 (ΔybdZ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATTTGCCATTCTATTTCTCC,  downstream forward: _UP4_TAATCTTATCAAAAAGAGAC
• BKK31959 (ΔybdZ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATTTGCCATTCTATTTCTCC,  downstream forward: _UP4_TAATCTTATCAAAAAGAGAC

References

Research papers

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