maeN

168

Na⁺/malate symporter

Locus: BSU_31580

Molecular weight: 47.66 kDa

Isoelectric point: 8.41

Protein length: 448 aa

Gene length: 1347 bp

Function: malate uptake

Product: Na⁺/malate symporter

Essential: no

Synonyms: maeN, yufR

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG3493 (Galperin et al., 2021)

Gene

Coordinates: 3,244,770 → 3,246,116

The protein

Protein family

• sodium:citrate (SCF) symporter family (together with CimH, according to UniProt)

Structure

• 5X9R (PDB) (from Klebsiella pneumoniae, 34% identity) PubMed
• O05256 (AlphaFold)

Paralogous protein(s)

• CimH

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: maeN

  Description: PubMed

  Regulation

  • induced by malate (MalR)

  Regulatory mechanism

  • MalR: activation, in malR regulon

  Additional information

  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

Mutant

• MGNA-B556 (yufR::erm), available at the NBRP B. subtilis, Japan
• 1A642 (maeN::Tn917, erm) (available from the BGSC or in Jörg Stülke's lab)
• 1A642 ( maeN::erm), PubMed, available at BGSC
• GP1449 (erm), available in Stülke lab
• BKE31580 (ΔmaeN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTCATACACCCCCATGT,  downstream forward: _UP4_TAAACAGAAAGAGTTCCGTT
• BKK31580 (ΔmaeN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTCATACACCCCCATGT,  downstream forward: _UP4_TAAACAGAAAGAGTTCCGTT

Expression vectors

• expression of native maeN in B. subtilis: pGP1951 (in pBQ200), available in Stülke lab

References

Reviews

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Original Publications

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