yugF

168

similar to dihydrolipoamide S-acetyltransferase

Locus: BSU_31420

Molecular weight: 31.18 kDa

Isoelectric point: 8.36

Protein length: 273 aa

Gene length: 822 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yugF

Genomic Context

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Categories containing this gene/protein

Lifestyles, Sporulation, Sporulation proteins, Newly identified sporulation proteins (based on transcription profiling)

Groups of genes, Poorly characterized/ putative enzymes

List of homologs in different organisms, belongs to COG0596 (Galperin et al., 2021)

Gene

Coordinates: 3,227,581  3,228,402

The protein

Protein family

dmpD/todF/xylF esterase family (single member, according to UniProt)

Domains

AB hydrolase-1 domain (aa 29-131) (according to UniProt)

Structure

5JZ9 (PDB) (26% identity) PubMed
O05235 (AlphaFold)

Expression and Regulation

Operons

Genes: yugF

Description: PubMed

Regulation

expressed during Sporulation PubMed

Biological materials

Mutant

MGNA-B550 (yugF::erm), available at the NBRP B. subtilis, Japan
BKE31420 (yugF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTGTGTCACTCCCTTT,  downstream forward: _UP4_TAACAAAAAAACCGGTGCTG
BKK31420 (yugF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTGTGTCACTCCCTTT,  downstream forward: _UP4_TAACAAAAAAACCGGTGCTG

References

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