tgl

168

outer spore coat protein, spore associated transglutaminase

Locus: BSU_31270

Molecular weight: 28.15 kDa

Isoelectric point: 6.69

Protein length: 245 aa

Gene length: 738 bp

Function: introduction of crosslinks in the spore coat proteins GerQ and SafA

Product: spore associated transglutaminase

Essential: no

E.C.: 2.3.2.13

Synonyms: tgl, yuxF, yugV

Genomic Context

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List of homologs in different organisms, belongs to COG5915 (Galperin et al., 2021)

Gene

Coordinates: 3,212,591  3,213,328

The protein

Catalyzed reaction/ biological activity

• cross-links SafA to other spore coat proteins PubMed
• L-glutaminyl-[protein] + L-lysyl-[protein] --> [protein]-L-lysyl-N⁶-5-L-glutamyl-[protein] + NH₄⁺ (according to UniProt)
• temperature-dependent modification of the coat proteins such as GerQ (together with YabG) PubMed

Protein family

• bacillus TGase family (single member, according to UniProt)

Structure

• 4P8I (PDB) PubMed
• 4PA5 (PDB) (complexed with cystamine) PubMed
• P40746 (AlphaFold)

Localization

• outer spore coat, localization depends on CotE PubMed
• inner spore coat and cortex, dependent on SafA PubMed

Expression and Regulation

Operons

• Genes: tgl

  Description: PubMed

  Regulation

  • expressed late during sporulation in the mother cell (SigE, SigK, GerE) PubMed

  Regulatory mechanism

  • GerE: repression, PubMed, in gerE regulon

  Sigma factors

  • SigK: sigma factor, PubMed, in sigK regulon
  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• MGNA-A615 (tgl::erm), available at the NBRP B. subtilis, Japan
• 1S122 ( tgl::erm), PubMed, available at BGSC
• BKE31270 (tgl::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTAGCCCCCCTTGTT,  downstream forward: _UP4_ATCGTCCGCTAAAAAGCCCC
• BKK31270 (tgl::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTAGCCCCCCTTGTT,  downstream forward: _UP4_ATCGTCCGCTAAAAAGCCCC

Labs working on this gene/protein

• Adriano Henriques, Lisbon, Portugal homepage

References

Reviews

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Original Publications

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