gbsB

168

choline dehydrogenase (FAD-dependent), glycine betaine synthesis

Locus: BSU_31050

Molecular weight: 43.39 kDa

Isoelectric point: 4.91

Protein length: 403 aa

Gene length: 1209 bp

Function: osmoprotection

Product: choline dehydrogenase (FAD-dependent)

Essential: no

E.C.: 1.1.1.1

Synonyms: gbsB

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1454 (Galperin et al., 2021)

Gene

Coordinates: 3,183,867 → 3,185,075

The protein

Catalyzed reaction/ biological activity

• choline + NAD⁺ --> betaine aldehyde + H⁺ + NADH (according to UniProt)

Protein family

• iron-containing alcohol dehydrogenase family (with YugJ and YugK, according to UniProt)

Cofactors

• FAD

Structure

• 3BFJ (PDB) (from Klebsiella pneumoniae, 39% identity) PubMed
• P71017 (AlphaFold)

Expression and Regulation

Operons

• Genes: gbsA-gbsB

  Description: PubMed

  Regulation

  • induced by choline (GbsR) PubMed

  Regulatory mechanism

  • GbsR: repression, (transcriptional roadblock) PubMed, in gbsR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• 1A1056 ( gbsB::kan), PubMed, available at BGSC
• BKE31050 (ΔgbsB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAATGTCATCATCAATCTCC,  downstream forward: _UP4_TAATCAAAATCCCGCTCCTT
• BKK31050 (ΔgbsB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAATGTCATCATCAATCTCC,  downstream forward: _UP4_TAATCAAAATCCCGCTCCTT

Labs working on this gene/protein

• Erhard Bremer, University of Marburg, Germany homepage

References

Loading