yuaF

168

NfeD2, role in maintaining membrane integrity during conditions of cellular stress, confers (together with FloT) resistance to cefuroxime

Locus: BSU_31020

Molecular weight: 18.46 kDa

Isoelectric point: 4.04

Protein length: 174 aa

Gene length: 525 bp

Function: involved in the control of membrane fluidity

Product: NfeD family protein NfeD2

Essential: no

Synonyms: yuaF, NfeD2

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms

Gene

Coordinates: 3,182,015 → 3,182,539

The protein

Catalyzed reaction/ biological activity

• SigW-dependent expression of ''fabF and the yuaF-floT-yuaI'' operon result in reduced membrane fluidity PubMed

Protein family

• NfeD family (with YqeZ)

Structure

• 2K14 (PDB) PubMed
• O32077 (AlphaFold)

Localization

• cell membrane, in focal structures PubMed

Expression and Regulation

Operons

• Genes: yuaF-floT-yuaI

  Description: PubMed

  Regulation

  • expressed upon cell wall stress (SigW) PubMed

  Sigma factors

  • SigW: sigma factor, PubMed, in sigW regulon

Biological materials

Mutant

• MGNA-A212 (yuaF::erm), available at the NBRP B. subtilis, Japan
• BKE31020 (ΔyuaF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACTTCCTCATCCTTTCTT,  downstream forward: _UP4_TAACATAAAAGGAGGAATTT
• BKK31020 (ΔyuaF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACTTCCTCATCCTTTCTT,  downstream forward: _UP4_TAACATAAAAGGAGGAATTT

References

Loading