thiT

168

S protein of thiamine ECF transporter

Locus: BSU_30990

Molecular weight: 20.31 kDa

Isoelectric point: 10.24

Protein length: 192 aa

Gene length: 579 bp

Function: thiamine uptake

Product: S protein of thiamine ECF transporter

Essential: no

Synonyms: thiT, yuaJ

Genomic Context

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List of homologs in different organisms, belongs to COG3859 (Galperin et al., 2021)

Gene

Coordinates: 3,179,306  3,179,884

The protein

Protein family

• vitamin uptake transporter (VUT/ECF) (TC 2.A.88) family (with TrpP and YpdP, according to UniProt)

Structure

• 3RLB (PDB) (from Lactococcus lactis, 32% identity) PubMed
• O32074 (AlphaFold)

Localization

• membrane PubMed

Expression and Regulation

Operons

• Genes: thiT

  Description: PubMed

  Regulation

  • repressed by thiamine ([regulon|Thi-box|]) PubMed

  Regulatory mechanism

  • Thi-box: RNA switch, [pubmed|], in Thi-box regulon

Biological materials

Mutant

• GP4787 (trpC2 ΔthiT::neo), available in Jörg Stülke's lab
• MGNA-A222 (yuaJ::erm), available at the NBRP B. subtilis, Japan
• BKE30990 (thiT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCTATCTCCTTCCATT,  downstream forward: _UP4_TAAAAGTAACAATCCCCCAG
• BKK30990 (thiT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCTATCTCCTTCCATT,  downstream forward: _UP4_TAAAAGTAACAATCCCCCAG

References

Reviews

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Original Publications

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