glgB

168

1,4-alpha-glucan branching enzyme

Locus: BSU_30980

Molecular weight: 73.49 kDa

Isoelectric point: 6.24

Protein length: 627 aa

Gene length: 1884 bp

Function: glycogen biosynthesis

Product: 1,4-alpha-glucan branching enzyme

Essential: no

E.C.: 2.4.1.18

Synonyms: glgB

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0296 (Galperin et al., 2021)

Gene

Coordinates: 3,169,763  3,171,646

The protein

Catalyzed reaction/ biological activity

• Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain (according to UniProt)

Protein family

• glycosyl hydrolase 13 family (according to UniProt)

Structure

• 5GQU (PDB) (from Cyanothece sp., 46% identity) PubMed
• P39118 (AlphaFold)

Paralogous protein(s)

• AmyX

Expression and Regulation

Operons

• Genes: glgB-glgC-glgD-glgA-glgP

  Description: PubMed

  Regulation

  • expressed early during sporulation in the mother cell (SigE) PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• BKE30980 (glgB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTAATCATCCTTTCTGA,  downstream forward: _UP4_AAAAAAAGAGGAGAGATAAA
• BKK30980 (glgB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTAATCATCCTTTCTGA,  downstream forward: _UP4_AAAAAAAGAGGAGAGATAAA

References

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