glgP

168

glycogen phosphorylase

Locus: BSU_30940

Molecular weight: 91.56 kDa

Isoelectric point: 6.28

Protein length: 798 aa

Gene length: 2394 bp

Function: glycogen biosynthesis

Product: glycogen phosphorylase

Essential: no

E.C.: 2.4.1.1

Synonyms: glgP

Genomic Context

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List of homologs in different organisms, belongs to COG0058 (Galperin et al., 2021)

Gene

Coordinates: 3,163,735 → 3,166,131

The protein

Catalyzed reaction/ biological activity

• [(1→4)-α-D-glucosyl]_(n) + phosphate --> [(1→4)-α-D-glucosyl]_(n-1) + α-D-glucose 1-phosphate (according to UniProt)

Protein family

• glycogen phosphorylase family (single member, according to UniProt)

Cofactors

• PLP (according to UniProt)

Structure

• 1PYG (PDB) (from Oryctolagus cuniculus, 45% identity) PubMed
• P39123 (AlphaFold)

Modification

• phosphorylation on (Thr-291 OR Ser-294) PubMed

Expression and Regulation

Operons

• Genes: glgB-glgC-glgD-glgA-glgP

  Description: PubMed

  Regulation

  • expressed early during sporulation in the mother cell (SigE) PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• BKE30940 (ΔglgP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAAAAATAAGTCTGCAAAGC,  downstream forward: _UP4_TGAAAAAGCCGCTGTATAAG
• BKK30940 (ΔglgP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAAAAATAAGTCTGCAAAGC,  downstream forward: _UP4_TGAAAAAGCCGCTGTATAAG

References

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