ytcA

168

similar to to UDP-sugar dehydrogenase

Locus: BSU_30860

Molecular weight: 46.67 kDa

Isoelectric point: 5.94

Protein length: 428 aa

Gene length: 1287 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: ytcA

Genomic Context

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List of homologs in different organisms, belongs to COG1004 (Galperin et al., 2021)

Gene

Coordinates: 3,155,725  3,157,011

The protein

Catalyzed reaction/ biological activity

• H₂O + 2 NAD⁺ + UDP-α-D-glucose --> 3 H⁺ + 2 NADH + UDP-α-D-glucuronate (according to UniProt)

Protein family

• UDP-glucose/GDP-mannose dehydrogenase family (with TuaD and Ugd, according to UniProt)

Structure

• 4A7P (PDB) (from Sphingomonas elodea, 39% identity)
• O34862 (AlphaFold)

Paralogous protein(s)

• TuaD, Ugd

Expression and Regulation

Operons

• Genes: ytcA-ytcB-ytcC

  Description: PubMed

  Regulation

  • expressed late during sporulation in the mother cell (SigKC, GerE) PubMed

  Regulatory mechanism

  • GerE: repression, PubMed, in gerE regulon

  Sigma factors

  • SigK: sigma factor, PubMed, in sigK regulon

Biological materials

Mutant

• MGNA-A280 (ytcA::erm), available at the NBRP B. subtilis, Japan
• BKE30860 (ytcA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCACCTGAGCTCCTTTAC,  downstream forward: _UP4_ATCTGTACGGGAGTTGGCCG
• BKK30860 (ytcA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCACCTGAGCTCCTTTAC,  downstream forward: _UP4_ATCTGTACGGGAGTTGGCCG

References

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