ytxM

168

similar to prolyl aminopeptidase

Locus: BSU_30810

Molecular weight: 30.55 kDa

Isoelectric point: 7.18

Protein length: 274 aa

Gene length: 825 bp

Function: ubiquinone and menaquinone biosynthesis

Product: unknown

Essential: no

E.C.: 4.2.99.20

Synonyms: ytxM, ytfB

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0596 (Galperin et al., 2021)

Gene

Coordinates: 3,149,751  3,150,575

The protein

Catalyzed reaction/ biological activity

• 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate --> (1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylate + pyruvate (according to UniProt)

Protein family

• AB hydrolase superfamily (according to UniProt)

Domains

• AB hydrolase-1 domain (aa 26-259) (according to UniProt)

Structure

• 2XMZ (PDB) (from Staphylococcus aureus, 34% identity) PubMed
• P23974 (AlphaFold)

Expression and Regulation

Operons

• Genes: menF-menD-ytxM-menB-menE-menC

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • A ncRNA is predicted between 'mntA' and 'menC' PubMed

Biological materials

Mutant

• MGNA-A283 (ytxM::erm), available at the NBRP B. subtilis, Japan
• BKE30810 (ytxM::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GACACCATCTGATACCGTTA,  downstream forward: _UP4_TGACTCATTCACATAGAGAA
• BKK30810 (ytxM::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GACACCATCTGATACCGTTA,  downstream forward: _UP4_TGACTCATTCACATAGAGAA

References

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