ytrF

168

ABC transporter (substrate-binding lipoprotein)

Locus: BSU_30410

Molecular weight: 48.26 kDa

Isoelectric point: 8.29

Protein length: 436 aa

Gene length: 1311 bp

Function: unknown

Product: ABC transporter  (substrate-binding lipoprotein)

Essential: no

Synonyms: ytrF

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG4591 (Galperin et al., 2021)

Gene

Coordinates: 3,113,979  3,115,289

The protein

Protein family

• ABC-4 integral membrane protein family (according to UniProt)

Structure

• O35005 (AlphaFold)

Localization

• cell membrane (via YtrC-YtrD) PubMed

Expression and Regulation

Operons

• Genes: ytrG-ytrA-ytrB-ytrC-ytrD-ytrE-ytrF

  Description: PubMed

  Regulation

  • expressed early in the stationary phase PubMed

  Regulatory mechanism

  • YtrA: repression, PubMed, in ytrA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• GP2700 (ΔytrF::cat), available in Jörg Stülke's lab PubMed
• GP3192 (ΔytrF), available in Jörg Stülke's lab PubMed
• GP2646 Δ(ytrG-ytrA-ytrB-ytrC-ytrD-ytrE-ytrF)::ermC, available in Jörg Stülke's lab PubMed
• MGNA-A132 (ytrF::erm), available at the NBRP B. subtilis, Japan
• BKE30410 (ytrF::erm  trpC2) available at BGSC and in Jörg Stülke's lab,  PubMed, upstream reverse: _UP1_TCTTCTGATAAAATGAACCT,  downstream forward: _UP4_TAATGCGAACGAGCCGGCTG
• BKK30410 (ytrF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TCTTCTGATAAAATGAACCT,  downstream forward: _UP4_TAATGCGAACGAGCCGGCTG

References

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