yteR

168

galacturonyl hydrolase, catalyses intracellular degradation of disaccharides generated by YesX

Locus: BSU_30120

Molecular weight: 42.81 kDa

Isoelectric point: 5.87

Protein length: 370 aa

Gene length: 1113 bp

Function: intracellular degradation of disaccharides generated by YesX

Product: galacturonyl hydrolase, catalyses intracellular degradation of disaccharides generated by YesX

Essential: no

Synonyms: yteR

Genomic Context

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List of homologs in different organisms, belongs to COG4225 (Galperin et al., 2021)

Gene

Coordinates: 3,081,131  3,082,243

The protein

Protein family

• glycosyl hydrolase 105 family (with RhiN, according to UniProt)

Structure

• 2GH4 (PDB),  2D8L (PDB) (complex with D-GlcA-Gal-nc)
• O34559 (AlphaFold)

Expression and Regulation

Operons

• Genes: yteP-yteR-yteS-yteT-yteU-yteV

  Description: PubMed

Additional information

• the mRNA is very stable (> 15 min) PubMed

Biological materials

Mutant

• MGNA-A811 (yteR::erm), available at the NBRP B. subtilis, Japan
• BKE30120 (yteR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTGATCCATTGATCCCATCC,  downstream forward: _UP4_CCTCTTTTTAGGTCAGCGGG
• BKK30120 (yteR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTGATCCATTGATCCCATCC,  downstream forward: _UP4_CCTCTTTTTAGGTCAGCGGG

References

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