pbuO

168

similar to hypoxanthine/guanine permease [protein|41DDD3AAFE89D0F52AB5ABD08CEE30A1767FFD7A|pbuG]

locus: BSU_29990

Molecular weight: 45.26 kDa

pI: 7.48

Protein length: 432 aa

Gene length: 1299 bp

function: unknown

product: unknown

essential: no

synonyms: pbuO, ytiP

List of homologs in different organisms, belongs to COG2252 (Galperin et al., 2021)

Gene

Coordinates: 3,068,908  3,070,206

The protein

Protein family

• [wiki|xanthine/uracil permease family] (according to UniProt)

Structure

• [AF|O34978]

Paralogous protein(s)

• [protein|41DDD3AAFE89D0F52AB5ABD08CEE30A1767FFD7A|pbuG]

Expression and Regulation

Operons

• genes: [gene|62AC66725C55B9E5FBBEB4EC43A88F7081B74D5C|pbuO]

  description: [Pubmed|9387221]

  regulation

  • repressed in the presence of purine nucleotides ([protein|search|PurR]) [Pubmed|11591660,2536750]

  regulatory mechanism

  • [protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]: repression, (molecular inducer: PRPP) [Pubmed|11591660,2536750], in [regulon|protein:A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR regulon]

Biological materials

Mutant

• MGNA-A819 (ytiP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/819 NBRP B. subtilis, Japan]
• BKE29990 ([gene|62AC66725C55B9E5FBBEB4EC43A88F7081B74D5C|pbuO]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29990 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGTATCCTCCAATTACG,  downstream forward: _UP4_TAAGGATAGACCAAAAAACC
• BKK29990 ([gene|62AC66725C55B9E5FBBEB4EC43A88F7081B74D5C|pbuO]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29990 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGTATCCTCCAATTACG,  downstream forward: _UP4_TAAGGATAGACCAAAAAACC

References

• 11591660,2536750,9387221