acuC

168

protein deacetylase for the control of AcsA activity

Locus: BSU_29710

Molecular weight: 42.83 kDa

Isoelectric point: 5.06

Protein length: 387 aa

Gene length: 1164 bp

Function: control of AcsA activity

Product: AcsA deacetylase

Essential: no

Synonyms: acuC

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0123 (Galperin et al., 2021)

Gene

Coordinates: 3,041,392  3,042,555

The protein

Catalyzed reaction/ biological activity

• deacetylates (and thereby activates) AcsA (together with SrtN)  PubMed

Protein family

• histone deacetylase family (single member, according to UniProt)

Structure

• 1C3R (PDB) (from Aquifex aeolicus, 35% identity) PubMed
• P39067 (AlphaFold)

Expression and Regulation

Operons

• Genes: acuA-acuB-acuC

  Description: PubMed

  Regulation

  • repressed by glucose (3.4-fold) (CcpA) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • the mRNA is quite stable (half-life 5 min) PubMed

Biological materials

Mutant

• GP1209 (''acuC::kan''), available in Jörg Stülke's lab
• GP1213 (''srtN::cat)(acuC::kan''), available in Jörg Stülke's lab
• 1A887 ( acuC::spc), PubMed, available at BGSC
• BKE29710 (acuC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TCTCATAGCAGATCCCTTTG,  downstream forward: _UP4_CAAAGAACAAAGTAAAAAAC
• BKK29710 (acuC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TCTCATAGCAGATCCCTTTG,  downstream forward: _UP4_CAAAGAACAAAGTAAAAAAC

References

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