acuA

168

protein acetylase for the control of AcsA and MenE activity

Locus: BSU_29690

Molecular weight: 24.18 kDa

Isoelectric point: 5.03

Protein length: 210 aa

Gene length: 633 bp

Function: control of AcsA and MenE activity

Product: Gcn5-related N-acetyltransferase

Essential: no

Synonyms: acuA

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 3,040,092  3,040,724

The protein

Catalyzed reaction/ biological activity

• acetylates (and thereby inactivates) AcsA on Lys-549 and MenE on Lys-471 PubMed

Protein family

• Acetyltransferase family (according to UniProt)
• Gcn5-relatedN-acetyltransferase  PubMed

Domains

• N-acetyltransferase domain (aa 20-161) (according to UniProt)

Structure

• 2Q04 (PDB) (from Exiguobacterium sibiricum, 59% identity)
• P39065 (AlphaFold)

Expression and Regulation

Operons

• Genes: acuA-acuB-acuC

  Description: PubMed

  Regulation

  • repressed by glucose (3.4-fold) (CcpA) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • the mRNA is quite stable (half-life 5 min) PubMed

Biological materials

Mutant

• GP1211 (acuA::kan), available in Jörg Stülke's lab
• GP1255 (''acuA::kan yjcK::cat ynaD::spec yoaA::tet''), available in Jörg Stülke's lab
• BKE29690 (acuA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTAATTCACCGTCCCATC,  downstream forward: _UP4_TAACTGACACTGACAAAGGG
• BKK29690 (acuA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTAATTCACCGTCCCATC,  downstream forward: _UP4_TAACTGACACTGACAAAGGG

References

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