ytrI

168

spore cortex formation

Locus: BSU_29240

Molecular weight: 19.75 kDa

Isoelectric point: 8.07

Protein length: 176 aa

Gene length: 531 bp

Function: spore cortex formation

Product: sporulation protein

Essential: no

Synonyms: ytrI

Genomic Context

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List of homologs in different organisms, belongs to COG5926 (Galperin et al., 2021)

Gene

Coordinates: 2,995,067 → 2,995,597

Phenotypes of a mutant

• ''pdaB ytrI'' double mutants are deficient in sporulation
• inactivation of ''ytrI'' reduces sporulation efficiency to 11.9% that of wild type cells PubMed

The protein

Structure

• O34460 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: ytrH-ytrI

  Description: PubMed

  Regulation

  • expressed early during sporulation in the mother cell (SigE) PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• MGNA-A430 (ytrI::erm), available at the NBRP B. subtilis, Japan
• BKE29240 (ΔytrI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GGTTTTTTGTAATGCTGGGG,  downstream forward: _UP4_TAATTATGGATTTTTTTTAA
• BKK29240 (ΔytrI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GGTTTTTTGTAATGCTGGGG,  downstream forward: _UP4_TAATTATGGATTTTTTTTAA

References

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