ytaF

168

sporulation protein

Locus: BSU_29070

Molecular weight: 17.23 kDa

Isoelectric point: 9.79

Protein length: 210 aa

Gene length: 633 bp

Function: unknown

Product: sporulation protein

Essential: no

Synonyms: ytaF

Genomic Context

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List of homologs in different organisms, belongs to COG1971 (Galperin et al., 2021)

Gene

Coordinates: 2,971,531  2,972,163

Phenotypes of a mutant

• strongly reduced sporulation efficiency (10% as compared to wild type) PubMed
• block in stage III of sporulation (engulfment of the forespore) PubMed

The protein

Domains

• 4 potential transmembrane domains PubMed

Structure

• C0SP79 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: polA-mutM-ytaF-coaE

  Description: PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• MGNA-A117 (ytaF::erm), available at the NBRP B. subtilis, Japan
• BKE29070 (ytaF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCCGTTTCTTCCTTTC,  downstream forward: _UP4_TAAAAAGGAGTGGATCTCTT
• BKK29070 (ytaF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCCGTTTCTTCCTTTC,  downstream forward: _UP4_TAAAAAGGAGTGGATCTCTT

References

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