lytS

168

two-component sensor kinase, control of pyruvate utilization

Locus: BSU_28930

Molecular weight: 64.68 kDa

Isoelectric point: 8.24

Protein length: 593 aa

Gene length: 1782 bp

Function: control of pyruvate utilization

Product: two-component sensor kinase

Essential: no

Synonyms: lytS

Genomic Context

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List of homologs in different organisms, belongs to COG3275 (Galperin et al., 2021)

Gene

Coordinates: 2,956,486  2,958,267

Phenotypes of a mutant

• no growth with pyruvate as the single carbon source PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + L-histidine --> ADP + N-phospho-L-histidine (according to UniProt)

Domains

• 5 transmembrane regions
• GAF domain (aa 238-363) (according to UniProt)
• Histidine kinase domain (aa 363-580) (according to UniProt)

Structure

• P94513 (AlphaFold)

Modification

• autophosphorylation on a His residue

Localization

• membrane (putative, due to presence of 5 membrane-spanning domains, according to Uniprot)

Expression and Regulation

Operons

• Genes: lytS-lytT

  Description: PubMed

Biological materials

Mutant

• MGNA-A015 (lytS::erm), available at the NBRP B. subtilis, Japan
• BKE28930 (lytS::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCATGTCACCTGTTCTT,  downstream forward: _UP4_GAACATGCTCAGGGTGTTAA
• BKK28930 (lytS::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCATGTCACCTGTTCTT,  downstream forward: _UP4_GAACATGCTCAGGGTGTTAA

References

Reviews

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Original Publications

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