lytT

168

two-component response regulator, control of pyruvate utilization

Locus: BSU_28920

Molecular weight: 27.68 kDa

Isoelectric point: 5.08

Protein length: 241 aa

Gene length: 726 bp

Function: control of pyruvate utilization

Product: two-component response regulator

Essential: no

Synonyms: lytT

Genomic Context

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List of homologs in different organisms, belongs to COG3279 (Galperin et al., 2021)

Gene

Coordinates: 2,955,783  2,956,508

The protein

Domains

• Response regulatory domain (aa 3-117) (according to UniProt)
• HTH LyTR-type domain (aa 137-241) (according to UniProt)

Structure

• P94514 (AlphaFold)

Modification

• phosphorylated on Asp by LytS

Localization

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• Genes: lytS-lytT

  Description: PubMed

Biological materials

Mutant

• MGNA-A014 (lytT::erm), available at the NBRP B. subtilis, Japan
• BKE28920 (lytT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCATCCCTTGCCAGCATCT,  downstream forward: _UP4_TGATGGGCGGCTTTTTGCAT
• BKK28920 (lytT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCATCCCTTGCCAGCATCT,  downstream forward: _UP4_TGATGGGCGGCTTTTTGCAT

References

Reviews

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Original Publications

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