ysdA

168

unknown

Locus: BSU_28840

Molecular weight: 10.18 kDa

Isoelectric point: 9.96

Protein length: 89 aa

Gene length: 270 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: ysdA

Genomic Context

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List of homologs in different organisms, belongs to COG3326 (Galperin et al., 2021)

Gene

Coordinates: 2,951,898  2,952,167

The protein

Structure

• P94519 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: infC-rpmI-rplT-ysdA

  Description: PubMed

  Regulation

  • Rel dependent downregulation (Class I) during stringent response PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response
  • RplT: termination, via binding to a RNA switch in the infC leader region causes transcription termination, in rplT regulon

  Additional information

  • autorepression of infC-rpmI-rplT-ysdA expression upon binding of excess RplT to the untranslated region of the mRNA PubMed
  • expression of IF3 is uncoupled from that of L35 and L20 by RNase Y-dependent mRNA processing PubMed
  • the RNA switch RNA is degraded by RNase Y PubMed

Biological materials

Mutant

• GP2391 ∆''ysdA::kan'', available in Jörg Stülke's lab
• MGNA-B024 (ysdA::erm), available at the NBRP B. subtilis, Japan
• BKE28840 (ysdA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTGCCTCATCCTTTAT,  downstream forward: _UP4_GATTTATAAAAAGCAGAAAA
• BKK28840 (ysdA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTGCCTCATCCTTTAT,  downstream forward: _UP4_GATTTATAAAAAGCAGAAAA

References

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