araA

168

L-arabinose isomerase

Locus: BSU_28800

Molecular weight: 56.37 kDa

Isoelectric point: 5.47

Protein length: 496 aa

Gene length: 1491 bp

Function: arabinose utilization

Product: L-arabinose isomerase

Essential: no

E.C.: 5.3.1.4

Synonyms: araA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2160 (Galperin et al., 2021)

Gene

Coordinates: 2,947,385  2,948,875

The protein

Catalyzed reaction/ biological activity

• L-arabinose --> L-ribulose (according to UniProt)

Protein family

• arabinose isomerase family (single member, according to UniProt)

Structure

• 2AJT (PDB) (from Escherichia coli, 51% identity, 71% similarity)\tPubMed
• P94523 (AlphaFold)

Kinetic information

• k (cat) of 14,504 min(-1) and  k (cat)/K (m) of 121 min(-1) mM(-1) for L-arabinose PubMed

Expression and Regulation

Operons

• Genes: araA-araB-araD-araL-araM-araN-araP-araQ-abfA

  Description: PubMed

  Regulation

  • induced by arabinose (AraR) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon
  • AraR: repression, PubMed, in araR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

Mutant

• BKE28800 (araA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTAAACTGCCCCTTTCT,  downstream forward: _UP4_CTTCAAAAGTAGAGGGGGAT
• BKK28800 (araA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTAAACTGCCCCTTTCT,  downstream forward: _UP4_CTTCAAAAGTAGAGGGGGAT

References

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