Difference between revisions of "RnhC"
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU28620&redirect=T BSU28620] | ||
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rnhC.html] | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rnhC.html] | ||
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU28620&redirect=T BSU28620] | ||
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2D0B 2D0B] (complex with Mg2+, Geobacillus stearothermophilus , 47% identity), [http://www.rcsb.org/pdb/explore.do?structureId=2D0A 2D0A] (Geobacillus stearothermophilus, 47% identity) | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2D0B 2D0B] (complex with Mg2+, Geobacillus stearothermophilus , 47% identity), [http://www.rcsb.org/pdb/explore.do?structureId=2D0A 2D0A] (Geobacillus stearothermophilus, 47% identity) | ||
Revision as of 14:27, 2 April 2014
- Description: RNase HIII, endoribonuclease
| Gene name | rnhC |
| Synonyms | ysgB |
| Essential | no |
| Product | Mg2+-dependent RNase HIII |
| Function | endonucleolytic cleavage of RNA in RNA-DNA hybrid molecules |
| Gene expression levels in SubtiExpress: rnhC | |
| MW, pI | 33 kDa, 10.07 |
| Gene length, protein length | 939 bp, 313 aa |
| Immediate neighbours | zapA, pheT |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU28620
Phenotypes of a mutant
Database entries
- BsubCyc: BSU28620
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Endonucleolytic cleavage to 5'-phosphomonoester (according to Swiss-Prot)
- Protein family: RnhC subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- RnhC interacts with the RNA polymerase PubMed
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- BsubCyc: BSU28620
- Structure: 2D0B (complex with Mg2+, Geobacillus stearothermophilus , 47% identity), 2D0A (Geobacillus stearothermophilus, 47% identity)
- UniProt: P94541
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: BP430 (ΔrnhC::cat) available in Fabian Commichau's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Takashi Tadokoro, Shigenori Kanaya
Ribonuclease H: molecular diversities, substrate binding domains, and catalytic mechanism of the prokaryotic enzymes.
FEBS J: 2009, 276(6);1482-93
[PubMed:19228197]
[WorldCat.org]
[DOI]
(I p)
Original publications
