zapA

168

positive modulator of FtsZ Z ring assembly and stability

Locus: BSU_28610

Molecular weight: 9.73 kDa

Isoelectric point: 7.17

Protein length: 85 aa

Gene length: 258 bp

Function: control of Z-ring formation

Product: Z-ring-associated protein, member of the divisome

Essential: no

Synonyms: zapA, yshA

Genomic Context

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List of homologs in different organisms, belongs to COG3027 (Galperin et al., 2021)

Gene

Coordinates: 2,925,640 → 2,925,897

Phenotypes of a mutant

• zapA and ezrA mutations are synthetically lethal, this can be suppressed by a FtsZ-K86E mutant protein PubMed
• a whiA zapA double mutant grows poorly, and the cells are filamentous (due to a defect in Z ring assembly) PubMed
• the defects of the whiA zapA double mutant can be suppressed by inactivation of either gtaB, pgcA, or ugtP PubMed

The protein

Protein family

• ZapA family (single member, according to UniProt)

Structure

• 1W2E (PDB) (from Pseudomonas aeruginosa) PubMed
• P94542 (AlphaFold)

Localization

• colocalizes with the Z-ring PubMed

Expression and Regulation

Operons

• Genes: zapA-yshB-polX-rqcU-yshE

  Description: PubMed

  Regulation

  • constitutively expressed PubMed

Biological materials

Mutant

• MGNA-B027 (yshA::erm), available at the NBRP B. subtilis, Japan
• BKE28610 (ΔzapA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACGTTTCTCCTCCATTCC,  downstream forward: _UP4_CTTAAAGAAAAGGATTGAAC
• BKK28610 (ΔzapA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACGTTTCTCCTCCATTCC,  downstream forward: _UP4_CTTAAAGAAAAGGATTGAAC

References

Reviews

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Original Publications

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