Difference between revisions of "SdhC"

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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU28450&redirect=T BSU28450]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sdhCAB.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sdhCAB.html]
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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU28450&redirect=T BSU28450]
  
 
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1NEK 1NEK] (''E. coli'')
 
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1NEK 1NEK] (''E. coli'')

Revision as of 14:26, 2 April 2014

  • Description: succinate dehydrogenase (cytochrome b558 subunit)

Gene name sdhC
Synonyms
Essential no
Product succinate dehydrogenase (cytochrome b558 subunit)
Function TCA cycle
Gene expression levels in SubtiExpress: sdhC
Interactions involving this protein in SubtInteract: SdhC
Metabolic function and regulation of this protein in SubtiPathways:
sdhC
MW, pI 22 kDa, 9.831
Gene length, protein length 606 bp, 202 aa
Immediate neighbours sdhA, yslB
Sequences Protein DNA DNA_with_flanks
Genetic context
SdhC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SdhC expression.png















Categories containing this gene/protein

carbon core metabolism, membrane proteins

This gene is a member of the following regulons

FsrA regulon

The gene

Basic information

  • Locus tag: BSU28450

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: cytochrome b558 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): Fe
  • Effectors of protein activity:
    • Inhibited by 2-(n-Heptyl)-4-hydroxy-quinoline N-oxide PubMed
    • Activated by Cytochrome b558 PubMed

Database entries

  • Structure: 1NEK (E. coli)
  • KEGG entry: [3]
  • E.C. number: 1.3.99.1

Additional information

  • This enzyme is a trimer membrane-bound PubMed PubMed
    • One subunit is bound to citochrome b558, and this subunit is the one bound to the cytosolic side of the membrane PubMed PubMed
    • Another subunit is the flavoprotein one, required for FAD usage PubMed PubMed
    • The other subunit has an iron-sulphur domain necessary for the catalytic activity PubMed PubMed
  • extensive information on the structure and enzymatic properties of succinate dehydrogenase can be found at Proteopedia

Expression and regulation

  • Regulation:
    • SdhC is less expressed under conditions of extreme iron starvation (FsrA) PubMed
    • part of the iron sparing response (FsrA) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications