bofC

168

general stress protein, controls processing of pro-SigK by SpoIVFB

Locus: BSU_27750

Molecular weight: 19.15 kDa

Isoelectric point: 5.75

Protein length: 170 aa

Gene length: 513 bp

Function: control of processing of pro-SigK by SpoIVFB

Product: forespore regulator of the Sigma-K checkpoint

Essential: no

E.C.: null

Synonyms: bofC

Genomic Context

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List of homologs in different organisms, belongs to COG5849 (Galperin et al., 2021)

Gene

Coordinates: 2,836,909  2,837,421

The protein

Structure

• 2BW2 (PDB)
• O05391 (AlphaFold)

Expression and Regulation

Operons

• Genes: csbX-bofC

  Description: PubMed

  Regulation

  • expressed during sporulation in the forespore (SigF, SigG) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigF: sigma factor, PubMed, in sigF regulon
  • SigG: sigma factor, PubMed, in sigG regulon
  • SigB: sigma factor, PubMed, in sigB regulon

Biological materials

Mutant

• BKE27750 (bofC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACCCTCTACACCTCTTTGC,  downstream forward: _UP4_TAGCGTCCGCTGATTGAAGA
• BKK27750 (bofC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACCCTCTACACCTCTTTGC,  downstream forward: _UP4_TAGCGTCCGCTGATTGAAGA

References

Reviews

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Original Publications

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