yrvM

168

2-methylthio-N6-threonylcarbamoyladenosine cyclase, catalyzes ATP-dependent dehydration of 2-methylthio N6-threonylcarbamoyladenosine (t6A) to form 2-methylthio cyclic N6-threonylcarbamoyladenosine (ms2ct6A )

Locus: BSU_27540

Molecular weight: 28.02 kDa

Isoelectric point: 8.19

Protein length: 254 aa

Gene length: 765 bp

Function: tRNA modification

Product: 2-methylthio-N6-threonylcarbamoyladenosine cyclase

Essential: no

Synonyms: tcdA, yrvM

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1179 (Galperin et al., 2021)

Gene

Coordinates: 2,813,643  2,814,407

The protein

Catalyzed reaction/ biological activity

• catalyzes ATP-dependent dehydration of 2-methylthio N6-threonylcarbamoyladenosine (t6A) to form  2-methylthio cyclic N6-threonylcarbamoyladenosine (ms2ct6A )  PubMed

Protein family

• HesA/MoeB/ThiF family (with MoeB and ThiF, according to UniProt)

Structure

• 4D7A (PDB) (from E. coli, 40% identity) PubMed
• O32037 (AlphaFold)

Expression and Regulation

Operons

• Genes: bsrA-yrvM

  Description: PubMed

Biological materials

Mutant

• MGNA-A826 (yrvM::erm), available at the NBRP B. subtilis, Japan
• BKE27540 (yrvM::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGAACCTCACTCCACATT,  downstream forward: _UP4_TAATGCATAAAAACAGCCTT
• BKK27540 (yrvM::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGAACCTCACTCCACATT,  downstream forward: _UP4_TAATGCATAAAAACAGCCTT

References

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