forE2

168

essential subunit of formate:menadione oxidoreductase ForC2-ForE2

Locus: BSU_27230

Molecular weight: 17.37 kDa

Isoelectric point: 4.76

Protein length: 160 aa

Gene length: 483 bp

Function: unknown

Product: partner subunit of formate:menadione oxidoreductase ForC2-ForE2

Essential: no

E.C.: null

Synonyms: yrhD

Genomic Context

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Categories containing this gene/protein

Groups of genes, Poorly characterized/ putative enzymes

List of homologs in different organisms, belongs to COG2427 (Galperin et al., 2021)

Gene

Coordinates: 2,784,170  2,784,652

The protein

Protein family

DUF1641 family (with YjlC and ForE1)

Structure

O05396 (AlphaFold)

Expression and Regulation

Operons

Genes: forC2-forE2

Description: PubMed

Additional information

term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of yrhE PubMed

Biological materials

Mutant

MGNA-A150 (yrhD::erm), available at the NBRP B. subtilis, Japan
BKE27230 (forE2::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GGCCATTCGTTTTCCTCCTA,  downstream forward: _UP4_TAACAGAAAAACAGCCATCT
BKK27230 (forE2::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GGCCATTCGTTTTCCTCCTA,  downstream forward: _UP4_TAACAGAAAAACAGCCATCT

References

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