forC2

168

formate:menadione oxidoreductase (with ForE2), catalytic subunit

Locus: BSU_27220

Molecular weight: 108.57 kDa

Isoelectric point: 5.16

Protein length: 980 aa

Gene length: 2943 bp

Function: unknown

Product: formate:menadione oxidoreductase

Essential: no

E.C.: 1.2.1.2

Synonyms: yrhE

Genomic Context

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List of homologs in different organisms, belongs to COG3383 (Galperin et al., 2021)

Gene

Coordinates: 2,781,209  2,784,151

The protein

Catalyzed reaction/ biological activity

• formate + NAD⁺ --> CO₂ + NADH (according to UniProt)

Protein family

• C-terminal part: prokaryotic molybdopterin-containing oxidoreductase family (according to UniProt)

Domains

• 2Fe-2S ferredoxin-type domain (aa 5-81) (according to UniProt)
• 4Fe-4S His(Cys)3-ligated-type domain (aa 81-121) (according to UniProt)
• 2 4Fe-4S ferredoxin-type domains (aa 144-171, aa 187-216) (according to UniProt)
• 4Fe-4S Mo/W bis-MGD-type domain (aa 263-319) (according to UniProt)

Cofactors

• 4 4Fe-4S cluster PubMed
• 1 2FE-2S cluster PubMed
• molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) PubMed
• menachinone-7 PubMed

Structure

• 2FUG (PDB) (from Thermus thermophilus, 23% identity) PubMed
• Q795Y4 (AlphaFold)

Modification

• Fe-S cluster PubMed

Paralogous protein(s)

• ForC1

Expression and Regulation

Operons

• Genes: forC2-forE2

  Description: PubMed

  Additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of yrhE PubMed

Biological materials

Mutant

• MGNA-A160 (yrhE::erm), available at the NBRP B. subtilis, Japan
• BKE27220 (forC2::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTCATATTCCCCTCCA,  downstream forward: _UP4_GATTAAACGATAGGAGGAAA
• BKK27220 (forC2::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTCATATTCCCCTCCA,  downstream forward: _UP4_GATTAAACGATAGGAGGAAA

References

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