yrhF

168

unknown

Locus: BSU_27210

Molecular weight: 13.84 kDa

Isoelectric point: 6.09

Protein length: 122 aa

Gene length: 369 bp

Function: unknown

Product: unknown

Essential: no

E.C.: null

Synonyms: yrhF

Genomic Context

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List of homologs in different organisms, belongs to COG5609 (Galperin et al., 2021)

Gene

Coordinates: 2,780,525 → 2,780,893

The protein

Structure

• O05398 (AlphaFold)

Expression and Regulation

Operons

• Genes: yrhF

  Description: PubMed

  Additional information

  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

• Genes: yrhF-yrhG-S1027-S1026-spyT-spyA-S1024

  Description: PubMed

  Additional information

  • the mRNA is cleaved by Rae1 within the spyA ORF PubMed
  • the mRNA is cleaved by RNase Y upstream of S1026 and further degraded by RNase J1to just upstream of the ribosomal binding site of spyT PubMed

Biological materials

Mutant

• MGNA-A149 (yrhF::erm), available at the NBRP B. subtilis, Japan
• BKE27210 (ΔyrhF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTGATTACCCCTTCCG,  downstream forward: _UP4_TAGTATATTTGTCATTGACA
• BKK27210 (ΔyrhF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTGATTACCCCTTCCG,  downstream forward: _UP4_TAGTATATTTGTCATTGACA

References

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