yraF

168

forespore-specific sporulation protein, similar to spore coat protein

locus: BSU_26960

Molecular weight: 13.87 kDa

pI: 5.14

Protein length: 122 aa

Gene length: 369 bp

function: unknown

product: unknown

essential: no

ec: null

synonyms: yraF

List of homologs in different organisms, belongs to COG5577 (Galperin et al., 2021)

Gene

Coordinates: 2,753,065  2,753,433

The protein

Protein family

• [wiki|CotF family] (according to UniProt)

Structure

• [AF|O07949]

[wiki|Localization]

• spore wall (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|7D61DB3A298C253BBC1C7B3AE169F8AAAE4610BB|yraG]-[gene|30A4306899B586898BC5B3666AD43D9DEE0579E1|yraF]-[gene|EA4CC0D36C39957DBBF9B1B11F65F33E8CD81FFE|adhB]-[gene|1D9D20DA24C13EF450710E8EDC5A36311DAF1BF0|yraE]-[gene|0D99D9ECB3BCD6E3F58F8CE8E8CC5AF718C1ECD8|yraD]

  description: [Pubmed|16497325]

  regulation

  • expressed during sporulation in the forespore ([protein|search|SigG]) [Pubmed|16497325]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|16497325], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]

Biological materials

Mutant

• MGNA-A028 (yraF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/28 NBRP B. subtilis, Japan]
• BKE26960 ([gene|30A4306899B586898BC5B3666AD43D9DEE0579E1|yraF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE26960 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATTCAAAGAGGCTTACCTCC,  downstream forward: _UP4_CACTAACACTGGAGGTTTTA
• BKK26960 ([gene|30A4306899B586898BC5B3666AD43D9DEE0579E1|yraF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK26960 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATTCAAAGAGGCTTACCTCC,  downstream forward: _UP4_CACTAACACTGGAGGTTTTA

References

• 16497325