yraH

168

unknown

Locus: BSU_26940

Molecular weight: 14.99 kDa

Isoelectric point: 4.99

Protein length: 128 aa

Gene length: 387 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yraH

Genomic Context

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List of homologs in different organisms, belongs to COG0346 (Galperin et al., 2021)

Gene

Coordinates: 2,752,167 → 2,752,553

The protein

Protein family

• glyoxalase I family (with GlxA and GlxB, according to UniProt)

Domains

• VOC domain (aa 2-127) (according to UniProt)

Structure

• 3EY7 (PDB) (from Vibrio cholerae, 27% identity)
• O07918 (AlphaFold)

Expression and Regulation

Operons

• Genes: yraH

  Description: PubMed

Biological materials

Mutant

• MGNA-A235 (yraH::erm), available at the NBRP B. subtilis, Japan
• BKE26940 (ΔyraH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCGAGTGCCTCCTATA,  downstream forward: _UP4_TGATTTTTTGACTCGCAAAA
• BKK26940 (ΔyraH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCGAGTGCCTCCTATA,  downstream forward: _UP4_TGATTTTTTGACTCGCAAAA