yrdN

168

putative 4-oxalocrotonate tautomerase

Locus: BSU_26660

Molecular weight: 14.54 kDa

Isoelectric point: 5.01

Protein length: 129 aa

Gene length: 390 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yrdN

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 2,725,114  2,725,503

The protein

Protein family

• 4-oxalocrotonate tautomerase family (according to UniProt)

Structure

• 2AAG (PDB) (from Pseudomonas pavonaceae 54% identity)
• P94502 (AlphaFold)

Paralogous protein(s)

• YodA

Biological materials

Mutant

• MGNA-A862 (yrdN::erm), available at the NBRP B. subtilis, Japan
• BKE26660 (yrdN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAAATCCCCTCCTTCTA,  downstream forward: _UP4_TAGTTGAATACTACCCTCAA
• BKK26660 (yrdN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAAATCCCCTCCTTCTA,  downstream forward: _UP4_TAGTTGAATACTACCCTCAA