blt

168

spermidine-efflux transporter

Locus: BSU_26590

Molecular weight: 43.26 kDa

Isoelectric point: 10.06

Protein length: 400 aa

Gene length: 1203 bp

Function: spermidine export

Product: spermidine-efflux transporter

Essential: no

Synonyms: blt, acfA, bmr2, bmt

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2814 (Galperin et al., 2021)

Gene

Coordinates: 2,716,973 → 2,718,175

The protein

Protein family

• major facilitator superfamily (according to UniProt)
• TCR/Tet family (according to UniProt)

Structure

• P39843 (AlphaFold)

Paralogous protein(s)

• Bmr

Expression and Regulation

Operons

• Genes: blt-bltD

  Description: PubMed

  Regulation

  • induced in the presence of polyamines PubMed

  Regulatory mechanism

  • BltR: activation, PubMed, in bltR regulon
  • Mta: activation, PubMed, in mta regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE26590 (Δblt::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTGAATTCCCCTTTTAC,  downstream forward: _UP4_TAATTCATTTTCTATAAAGT
• BKK26590 (Δblt::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTGAATTCCCCTTTTAC,  downstream forward: _UP4_TAATTCATTTTCTATAAAGT

References

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