yrkC

168

mother cell-specific sporulation protein

Locus: BSU_26560

Molecular weight: 21.11 kDa

Isoelectric point: 7.35

Protein length: 186 aa

Gene length: 561 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yrkC

Genomic Context

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List of homologs in different organisms, belongs to COG0662 (Galperin et al., 2021)

Gene

Coordinates: 2,714,933 → 2,715,493

The protein

Domains

• cupin 2 domain (aa 89 ... 164)

Structure

• 2OA2 (PDB) (the cupin 2 domain, from B. halodurans, 66% identity)
• P54430 (AlphaFold)

Expression and Regulation

Operons

• Genes: yrkC-yrzN

  Description: PubMed

  Regulation

  • expressed late during sporulation in the mother cell (SigK) PubMed

  Sigma factors

  • SigK: sigma factor, PubMed, in sigK regulon

Biological materials

Mutant

• MGNA-C456 (yrkC::erm), available at the NBRP B. subtilis, Japan
• BKE26560 (ΔyrkC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTTGATCCCCGCAAT,  downstream forward: _UP4_TAGCACAGATATTCTGGAGA
• BKK26560 (ΔyrkC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTTTGATCCCCGCAAT,  downstream forward: _UP4_TAGCACAGATATTCTGGAGA

References

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