sigKC

168

RNA polymerase sporulation-specific sigma factor (SigK) (3' part of the interrupted sigK gene), with sigK

Locus: BSU_26390

Molecular weight: 16.00 kDa

Isoelectric point: 9.608

Protein length: 138 aa

Gene length: 417 bp

Function: late mother cell-specific gene expression

Product: RNA polymerase sporulation-specific sigma factor (SigK) (3' part of the interrupted sigK gene)

Essential: no

Synonyms: sigKC, spoIIIC

Genomic Context

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Gene

Coordinates: 2,701,338  2,701,754

The protein

Protein family

• sigma-70 factor family (according to UniProt)

Structure

• P12254 (AlphaFold)

Expression and Regulation

Operons

• Genes: sigKC

  Description: PubMed

  Regulation

  • expressed during sporulation in the mother cell (SigE, SigK, [protein|[GerE]], SpoIIID) PubMed

  Regulatory mechanism

  • GerE: repression, PubMed, in gerE regulon
  • SpoIIID: activation, PubMed, in spoIIID regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon
  • SigK: sigma factor, PubMed, in sigK regulon

• Genes: yqaB-sigKC

  Description: PubMed

Biological materials

Mutant

• BKE26390 (sigKC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATGCATGAGGATTTCATTAT,  downstream forward: _UP4_TAATGCAGAAGAAGCCGGAT
• BKK26390 (sigKC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATGCATGAGGATTTCATTAT,  downstream forward: _UP4_TAATGCAGAAGAAGCCGGAT

References

Other original publications

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The SigK regulon

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