cwlA

168

N-acetylmuramoyl-L-alanine amidase

Locus: BSU_25900

Molecular weight: 29.81 kDa

Isoelectric point: 10.4

Protein length: 272 aa

Gene length: 819 bp

Function: minor autolysin

Product: N-acetylmuramoyl-L-alanine amidase

Essential: no

E.C.: 3.5.1.28

Synonyms: cwlA

Genomic Context

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List of homologs in different organisms, belongs to COG5632 (Galperin et al., 2021)

Gene

Coordinates: 2,664,573 → 2,665,391

The protein

Catalyzed reaction/ biological activity

• Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)

Protein family

• N-acetylmuramoyl-L-alanine amidase 2 family (according to UniProt)

Domains

• contains an amidase_2 domain (like BlyA, CwlH, XlyA, XlyB)
• N-acetylmuramoyl-L-alanine amidase domain (aa 24-142) (according to UniProt)

Structure

• 2L47 (PDB) (from Bacillus phage Gamma, corresponds to the N-terminal domain, aa 3 ... 150, 61% identity)
• P24808 (AlphaFold)

Paralogous protein(s)

• CwlH, XlyA, XlyB

Biological materials

Mutant

• BKE25900 (ΔcwlA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATACCCTCTCTCCTTCT,  downstream forward: _UP4_TAAATAAATAGTCTCCTTGA
• BKK25900 (ΔcwlA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATACCCTCTCTCCTTCT,  downstream forward: _UP4_TAAATAAATAGTCTCCTTGA

References

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