cwlH

168

N-acetylmuramoyl-L-alanine amidase

Locus: BSU_25710

Molecular weight: 27.42 kDa

Isoelectric point: 10

Protein length: 250 aa

Gene length: 753 bp

Function: cell wall metabolism

Product: N-acetylmuramoyl-L-alanine amidase

Essential: no

E.C.: 3.5.1.28

Synonyms: cwlH, yqeE

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG5632 (Galperin et al., 2021)

Gene

Coordinates: 2,648,903 → 2,649,655

The protein

Catalyzed reaction/ biological activity

• Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)

Protein family

• N-acetylmuramoyl-L-alanine amidase 2 family (according to UniProt)

Domains

• contains an amidase_2 domain (like BlyA, CwlA, XlyA, XlyB)
• N-acetylmuramoyl-L-alanine amidase domain (aa 45-141) (according to UniProt)

Structure

• 3RDR (PDB) (N-terminal domain, aa 1 - 150, XlyA, 75% identity) PubMed
• 1LBU (PDB) (C-terminal peptidoglycan-binding domain, aa 183 - 245, 48% identity)
• P54450 (AlphaFold)

Paralogous protein(s)

• XlyA, XlyB, CwlA

Localization

• secreted (according to UniProt)

Expression and Regulation

Operons

• Genes: cwlH

  Description: PubMed

  Regulation

  • expressed late during sporulation in the mother cell (SigK, GerE) PubMed

  Regulatory mechanism

  • GerE: activation, PubMed, in gerE regulon

  Sigma factors

  • SigK: sigma factor, PubMed, in sigK regulon

Biological materials

Mutant

• BKE25710 (ΔcwlH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCTATCCCTCCCTATT,  downstream forward: _UP4_TAAATGCAGGATTTAACGGA
• BKK25710 (ΔcwlH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCTATCCCTCCCTATT,  downstream forward: _UP4_TAAATGCAGGATTTAACGGA

References

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