yqeM

168

similar to SAM-dependent methyltransferase

Locus: BSU_25610

Molecular weight: 28.18 kDa

Isoelectric point: 4.26

Protein length: 247 aa

Gene length: 744 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yqeM

Genomic Context

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List of homologs in different organisms, belongs to COG2226 (Galperin et al., 2021)

Gene

Coordinates: 2,642,101  2,642,844

The protein

Protein family

• Methyltransferase superfamily (according to UniProt)

Cofactors

• SAM

Structure

• 3D2L (PDB) (from Exiquobacterium sp., 45% identity)
• P54458 (AlphaFold)

Expression and Regulation

Operons

• Genes: pgpP-yqeH-aroD-yqeI-nadD-yqeK-yqeL-yqeM

  Description: PubMed

  Expression

  • expression is increased upon depletion of depletion of tRNA maturation factors rnpB or rnz PubMed

Biological materials

Mutant

• MGNA-C499 (yqeM::erm), available at the NBRP B. subtilis, Japan
• BKE25610 (yqeM::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGCTTGCAAAGCCTTGAT,  downstream forward: _UP4_TAAAACGATGGTTTTTTAAA
• BKK25610 (yqeM::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGCTTGCAAAGCCTTGAT,  downstream forward: _UP4_TAAAACGATGGTTTTTTAAA

References

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