comEA

168

membrane DNA receptor, plays an important role in the transfer of transforming DNA into the DNA channel and in controlling the rate of DNA uptake

Locus: BSU_25590

Molecular weight: 21.62 kDa

Isoelectric point: 4.82

Protein length: 205 aa

Gene length: 618 bp

Function: genetic competence

Product: membrane DNA receptor

Essential: no

E.C.: null

Synonyms: comEA, comD

Genomic Context

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List of homologs in different organisms, belongs to COG1555 (Galperin et al., 2021)

Gene

Coordinates: 2,640,513  2,641,130

Phenotypes of a mutant

• strongly reduced transformation rate PubMed

The protein

Catalyzed reaction/ biological activity

• stabilizes the attachment of transforming DNA at localized regions in the periplasm and then mediates uptake, probably by a Brownian ratchet mechanism PubMed

Domains

• TM domain: aa 10 - 30 PubMed
• extracellular DNA-binding domain: 2 HhH domains (aa 142-171,aa 172-302) (according to UniProt)

Structure

• 8DFK (PDB) PubMed
• P39694 (AlphaFold)

Localization

• cell membrane (integral), no distinct position PubMed
• localizes along the long axis of competent cells PubMed

Expression and Regulation

Operons

• Genes: comEA-comEB-comEC

  Description: PubMed

  Regulatory mechanism

  • ComK: activation, PubMed, in comK regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Other regulations

• ComN: unknown, PubMed

Biological materials

Mutant

• BKE25590 (comEA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGTGCATGTTCCCCGCT,  downstream forward: _UP4_TGATTTGACGGTCATGCATT
• BKK25590 (comEA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGTGCATGTTCCCCGCT,  downstream forward: _UP4_TGATTTGACGGTCATGCATT

References

Reviews

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Original Publications

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