comEC

168

channel protein for DNA binding and uptake

Locus: BSU_25570

Molecular weight: 86.51 kDa

Isoelectric point: 6.96

Protein length: 776 aa

Gene length: 2331 bp

Function: genetic competence

Product: DNA channel

Essential: no

Synonyms: comEC, comD

Genomic Context

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List of homologs in different organisms, belongs to COG2333 (Galperin et al., 2021)

Gene

Coordinates: 2,637,543  2,639,873

Phenotypes of a mutant

• loss of genetic competence PubMed

The protein

Catalyzed reaction/ biological activity

• extracellular degradation of one strand of the dsDNA and uptake of the remaining ssDNA molecule PubMed

Domains

• N-terminal transmembrane DNA-transport channel
• C-terminal extracellular nuclease domain PubMed

Cofactors

• Mn²⁺ (for nuclease activity) PubMed

Structure

• P39695 (AlphaFold)

Localization

• cell membrane PubMed
• localizes to one or both cell poles PubMed

Expression and Regulation

Operons

• Genes: comEA-comEB-comEC

  Description: PubMed

  Regulatory mechanism

  • ComK: activation, PubMed, in comK regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Other regulations

• ComN: unknown, PubMed

Additional information

• translation of ComEC is translationally coupled to expression of ComEB PubMed

Biological materials

Mutant

• GP2643 (ΔcomEC::spc) available in Jörg Stülke's lab PubMed
• BKE25570 (comEC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCATCACACGTAGCTCG,  downstream forward: _UP4_TAAAAAAGACTGCCGAGAAA
• BKK25570 (comEC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCATCACACGTAGCTCG,  downstream forward: _UP4_TAAAAAAGACTGCCGAGAAA

References

Reviews

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Original Publications

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